Microbes and microbial strategies in carcinogenic polycyclic aromatic hydrocarbons remediation: a systematic review.

Degradation, detoxification, or removal of the omnipresent polycyclic aromatic hydrocarbons (PAHs) from the ecosphere as well as their prevention from entering into food chain has never appeared simple. In this context, cost-effective, eco-friendly, and sustainable solutions like microbe-mediated strategies have been adopted worldwide. With this connection, measures have been taken by multifarious modes of microbial remedial strategies, i.e., enzymatic degradation, biofilm and biosurfactant production, application of biochar-immobilized microbes, lactic acid bacteria, rhizospheric-phyllospheric-endophytic microorganisms, genetically engineered microorganisms, and bioelectrochemical techniques like microbial fuel cell. In this review, a nine-way directional approach which is based on the microbial resources reported over the last couple of decades has been described. Fungi were found to be the most dominant taxa among the CPAH-degrading microbial community constituting 52.2%, while bacteria, algae, and yeasts occupied 37.4%, 9.1%, and 1.3%, respectively. In addition to these, category-wise CPAH degrading efficiencies of each microbial taxon, consortium-based applications, CPAH degradation-related molecular tools, and factors affecting CPAH degradation are the other important aspects of this review in light of their appropriate selection and application in the PAH-contaminated environment for better human-health management in order to achieve a sustainable ecosystem.

Augmented growth of Cd-stressed rice seedlings with the application of phytostimulating, root-colonizing, Cd-tolerant, leaf-endophytic fungi Colletotrichum spp. isolated from Eupatorium triplinerve.

The potential of plant growth-promoting endophytic fungi (PGPEF) in mycoremediation has received notable attention in recent years. Unlike other root-colonizing microorganisms, PGPEF colonization under Cadmium (Cd) stress is a less-revealed phenomenon. Among eighteen fungal isolates from the leaves of Eupatorium triplinerve, twelve were found as the species of Colletotrichum and remaining six belong to Fusarium based on phenotypic characterization. However, only two PGPEF isolates (ALE15 and ALE18) were finally selected based on possession of ACCD activity (~0.84 and 0.47 nM/µg protein/h, respectively) and higher Cd tolerance (1000 and 750 µg/mL, respectively). Moreover, the said isolates showed IAA production (~248 and 289 µg/mL), GA production (~86 and 88 AUs), phosphate solubilization (~165 and 256 µg/mL, respectively) under Cd stress. ALE18 strain was found to produce siderophore too. Molecular identification through sequencing of ITS region of both isolates confirmed their identity as species of Colletotrichum. Furthermore, FESEM-EDAX and AAS analyses supported their Cd bioaccumulation ability in mycelial cells that directly impacted to assist rice seedlings' (IR-36 cultivar) growth under Cd stress. Successful root colonization was also observed through FESEM and fluorescence microscopic studies. Finally, the detached leaf experiment with six economically important crops assured their applicability on field-scale as non-pathogenic PGPEF candidates.

Molecular insight into arsenic uptake, transport, phytotoxicity, and defense responses in plants: a critical review.

MAIN CONCLUSION: A critical investigation into arsenic uptake and transportation, its phytotoxic effects, and defense strategies including complex signaling cascades and regulatory networks in plants. The metalloid arsenic (As) is a leading pollutant of soil and water. It easily finds its way into the food chain through plants, more precisely crops, a common diet source for humans resulting in serious health risks. Prolonged As exposure causes detrimental effects in plants and is diaphanously observed through numerous physiological, biochemical, and molecular attributes. Different inorganic and organic As species enter into the plant system via a variety of transporters e.g., phosphate transporters, aquaporins, etc. Therefore, plants tend to accumulate elevated levels of As which leads to severe phytotoxic damages including anomalies in biomolecules like protein, lipid, and DNA. To combat this, plants employ quite a few mitigation strategies such as efficient As efflux from the cell, iron plaque formation, regulation of As transporters, and intracellular chelation with an array of thiol-rich molecules such as phytochelatin, glutathione, and metallothionein followed by vacuolar compartmentalization of As through various vacuolar transporters. Moreover, the antioxidant machinery is also implicated to nullify the perilous outcomes of the metalloid. The stress ascribed by the metalloid also marks the commencement of multiple signaling cascades. This whole complicated system is indeed controlled by several transcription factors and microRNAs. This review aims to understand, in general, the plant-soil-arsenic interaction, effects of As in plants, As uptake mechanisms and its dynamics, and multifarious As detoxification mechanisms in plants. A major portion of this article is also devoted to understanding and deciphering the nexus between As stress-responsive mechanisms and its underlying complex interconnected regulatory networks.

Structural heterogeneity assessment among the isoforms of fungal 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase: a comparative in silico perspective.

BACKGROUND: The primary amino acid sequence of a protein is a translated version from its gene sequence which carries important messages and information concealed therein. The present study unveils the structure-function and evolutionary aspects of 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) proteins of fungal origin. ACCD, an important plant growth-promoting microbial enzyme, is less frequent in fungi compared to bacteria. Hence, an inclusive understanding of fungal ACC deaminases (fACCD) has brought forth here. RESULTS: In silico investigation of 40 fACCD proteins recovered from NCBI database reveals that fACCD are prevalent in Colletotrichum (25%), Fusarium (15%), and Trichoderma (10%). The fACCD were found 16.18-82.47 kDa proteins having 149-750 amino acid residues. The enzyme activity would be optimum in a wide range of pH having isoelectric points 4.76-10.06. Higher aliphatic indices (81.49-100.13) and instability indices > 40 indicated the thermostability nature. The secondary structural analysis further validates the stability owing to higher α-helices. Built tertiary protein models designated as ACCNK1-ACCNK40 have been deposited in the PMDB with accessions PM0083418-39 and PM0083476-93. All proteins were found as homo-dimer except ACCNK13, a homo-tetramer. CONCLUSIONS: Hence, these anticipated features would facilitate to explore and identify novel variants of fungal ACCD in vitro aiming to industrial-scale applications.

A potent cadmium bioaccumulating Enterobacter cloacae strain displays phytobeneficial property in Cd-exposed rice seedlings.

In agricultural soil, cadmium (Cd) pollution compromises soil health, reduces crop yield, and produces Cd-contaminated crops. Bio-based approaches are necessary as an eco-friendly and sustainable solution to mitigate Cd-polluted areas. A heavy metal-resistant rhizobacterial strain (AS10) has been isolated from a heavy metal-defiled rice field. The 16S rDNA sequence and MALDI-TOF MS analyses of ribosomal protein reveal its identity closely similar to Enterobacter cloacae. The strain was found to withstand up to 4000 µg/ml Cd2+, 3312 µg/ml Pb2+ and 1500 µg/ml As3+. The Cd2+ removal efficiency was recorded as high as 72.11% when grown in 4000 µg/ml Cd2+. The strain's Cd-accumulation efficiency was also apprehended by TEM-EDAX followed by XRD-XRF-FTIR analyses. Besides, the strain showed solubilization of inorganic phosphate, ACC deaminase activity, nitrogen fixation and IAA production ability. Added further, the strain, as an efficient bioinoculant, significantly improved rice plant growth at the seedling stage through Cd immobilization. It prevented the surge of stress ethylene and oxidative stress in rice seedlings, resulting in overall plant growth improvement. Hence, the strain AS10 as potent plant growth-promoting rhizobacteria (PGPR) may be beneficial, especially in heavy metal-contaminated crop fields.

Electrochemical Column Cell for Continuous Oxidative Inactivation of Pathogens and Reductive Removal of Toxic Heavy Metals.

A solar-driven electrochemical column (EC) was developed for cathodic sequestration remediation of heavy metals (HMs) and anodic electroporative inactivation of pathogenic bacteria (PB) with continuous flow capacity for sustainable production of drinking water from wastewater. The method produces "revitalized drinking water" by keeping its natural mineral nutrients boosted with dissolved oxygen. The EC was constructed with graphene oxide (GO) synthesized via photoassisted electrochemical oxidation of CF (PEGO-CF) as the cathode and phytoreduced GO (RPEGO-CF) as the anode. In the EC, effluent is passed upward through the microchannel of CF electrodes to obtain a higher contact time with water molecules, enabling deposition of HMs and oxidative inactivation of PB, collectively termed electroadsorptive dialysis (EAD). PEGO-CF and RPEGO-CF stacked inside the EC resulted in the increased surface area and thereby the removal efficiency. Reactive oxygen species (ROS) produced at the anode damaged the bacterial cell sheath, while the oxygen functional group and the cathodic negative potential had a concurrent effect in "sequestration" of HMs. Density functional calculations showed that PEGO might transfer an electron from the highest occupied molecular orbital (HOMO) to the lowest unoccupied molecular orbital (LUMO) under applied negative potential leading to internal system crossing to the vacant d-orbitals of HMs, allowing for simultaneous coordination and deposition. The EC produced 313 L of revitalized water from wastewater augmented with 500 µg L-1 HMs and 107 CFU mL-1 pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Only a 3.6 J energy investment produced 1 L of revitalized water, which is ∼2000 times less than the usual energy consumption by electroporation and the lowest value obtained to date for bacterial inactivation with heavy metal removal. Laboratory-to-industrial scale-up calculations were performed for this water-purifying technology involving a water-energy nexus, promising high-efficiency bacterial inactivation, and HM remediation to obtain energy-efficient clean and revitalized water.

Unraveling the role of plant growth-promoting rhizobacteria in the alleviation of arsenic phytotoxicity: A review.

The toxic metalloid arsenic (As), is a major pollutant of soil and water, imposing severe health concerns on human lives. It enters the food chain mainly through As-contaminated crops. The uptake, translocation and accumulation of As in plant tissue are often controlled by certain soil-inhabiting microbial communities. Among them, indigenous, free-living As-resistant plant growth-promoting rhizobacteria (PGPR) plays a pivotal role in As-immobilization. Besides, the plant's inability to withstand As after a threshold level is actively managed by these PGPR increasing As-tolerance in host plants by a synergistic plant-microbe interaction. The dual functionality of As-resistant PGPR i.e., phytostimulation and minimization of As-induced phytotoxic damages are one of the main focal points of this review article. It is known that such PGPR having the functional arsenic-resistant genes (in ars operon) including As-transporters, As-transforming genes contributed to the As accumulation and detoxification/transformation respectively. Apart from assisting in nutrient acquisition and modulating phytohormone levels, As-resistant PGPR also influences the antioxidative defense system in plants by maneuvering multiple enzymatic and non-enzymatic antioxidants. Furthermore, they are effective in reducing membrane damage and electrolyte leakage in plant cells. As-induced photosynthetic damage is also found to be salvaged by As-resistant PGPR. Briefly, the eco-physiological, biochemical and molecular mechanisms of As-resistant PGPR are thus elaborated here with regard to the As-exposed crops.

Unraveling the heavy metal resistance and biocontrol potential of Pseudomonas sp. K32 strain facilitating rice seedling growth under Cd stress.

Heavy metal and metalloid toxicity in agricultural land needs special attention for crop production essential to feed increasing population globally. Plant growth-promoting rhizobacteria (PGPR) are native biological agents that have tremendous potential to augment crop production in contaminated fields. This study involves selection and identification (through 16S rRNA gene sequence and FAME analysis) of a potent Pseudomonas sp. (strain K32) isolated from a metal-contaminated rice rhizosphere, aimed to its application for sustainable agriculture. Apart from multi-heavy metal(loid) resistance (Cd2+, Pb2+ and As3+ upto 4000, 3800, 3700 µg/ml respectively) along with remarkable Cd bioaccumulation potential (∼90%), this strain showed IAA production, nitrogen-fixation and phosphate solubilization under Cd stress. This bioaccumulation efficiency coupled with PGP traits resulted in the significant enhancement of rice seedling growth under Cd stress. This positive impact of K32 strain was clearly manifested in morphological and biochemical improvements under Cd stress including successful root colonization with rice roots. Cd uptake was also reduced significantly in seedlings in presence of K32 strain. Together with all mentioned properties, K32 showed bio-control potential against plant pathogenic fungi viz. Aspergillus flavus, Aspergillus parasiticus, Paecilomyces sp., Cladosporium herbarum, Rhizopus stolonifer and Alternaria alternata which establish K32 strain a key player in effective bioremediation of agricultural fields. Biocontrol potential was found to be the result of enzymatic activities viz. chitinase, ß-1,3-glucanase and protease which were estimated as 8.17 ± 0.44, 4.38 ± 0.35 and 7.72 ± 0.28 U/mg protein respectively.

Abatement of arsenic-induced phytotoxic effects in rice seedlings by an arsenic-resistant Pantoea dispersa strain.

Population detonation and rapid industrialization are the major factors behind the reduction in cultivable land that affects crop production seriously. This situation is further being deteriorated due to the negative effects of abiotic stresses. Under such conditions, plant growth-promoting rhizobacteria (PGPR) are found to improve crop production which is essential for sustainable agriculture. This study is focused on the isolation of potent arsenic (As)-resistant PGPR from the agricultural land of West Bengal, India, and its application to reduce As translocation in rice seedlings. After screening, an As-resistant PGPR strain AS18 was identified by phenotypic characters and 16S rDNA sequence-based homology as Pantoea dispersa. This strain displayed nitrogen fixation, phosphate solubilization, 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) activity, indole-3-acetic acid (IAA) production, in addition to As (III) resistance up to 3750 µg/mL. The As removal efficiency of this strain was up to 93.12% from the culture medium as evidenced by AAS. The bioaccumulation property of AS18 strain was further validated by TEM-EDAX-XRD-XRF-FTIR studies. This strain showed significant morpho-biochemical improvements including antioxidant enzymatic activities and As-minimization in plant (rice) cells. Thus, being an As-resistant potent PGPR, AS18 strain is expected to be applied in As-spiked agricultural fields for bioremediation and phytostimulation.

Metagenomic Insights into Rhizospheric Microbiome Profiling in Lentil Cultivars Unveils Differential Microbial Nitrogen and Phosphorus Metabolism under Rice-Fallow Ecology.

The plant rhizosphere interfaces an array of microbiomes related to plant growth and development. Cultivar-specific soil microbial communities with respect to their taxonomic structure and specific function have not been investigated explicitly in improving the adaptation of lentil cultivars under rice-fallow ecology. The present study was carried out to decipher the rhizosphere microbiome assembly of two lentil cultivars under rice-fallow ecology for discerning the diversity of microbial communities and for predicting the function of microbiome genes related to nitrogen (N) and phosphorus (P) cycling processes deploying high-throughput whole (meta) genome sequencing. The metagenome profile of two cultivars detected variable microbiome composition with discrete metabolic activity. Cyanobacteria, Bacteroidetes, Proteobacteria, Gemmatimonadetes, and Thaumarchaeota were abundant phyla in the "Farmer-2" rhizosphere, whereas Actinobacteria, Acidobacteria, Firmicutes, Planctomycetes, Chloroflexi, and some incompletely described procaryotes of the "Candidatus" category were found to be robustly enriched the rhizosphere of "Moitree". Functional prediction profiles of the microbial metagenomes between two cultivars revealed mostly house keeping genes with general metabolism. Additionally, the rhizosphere of "Moitree" had a high abundance of genes related to denitrification processes. Significant difference was observed regarding P cycling genes between the cultivars. "Moitree" with a profuse root system exhibited better N fixation and translocation ability due to a good "foraging strategy" for improving acquisition of native P under the nutrient depleted rice-fallow ecology. However, "Farmer-2" revealed a better "mining strategy" for enhancing P solubilization and further transportation to sinks. This study warrants comprehensive research for explaining the role of microbiome diversity and cultivar-microbe interactions towards stimulating microbiome-derived soil reactions regarding nutrient availability under rice-fallow ecology.

Artificial Bifunctional Photozyme of Glucose Oxidase-Peroxidase for Solar-Powered Glucose-Peroxide Detection in a Biofluid with Resorcinol-Formaldehyde Polymers.

Photozymes or artificial photosynthesis based on alternative natural enzymes is vital for the sustainable development of next-generation healthcare, energy, and materials science. Herein, we report resorcinol-formaldehyde (RF) resins as a solar-driven metal-free bifunctional glucose oxidase-peroxidase stand-alone photozyme for the colorimetric dual detection of hydrogen peroxide and glucose. The π-bond conjugated benzenoid-ortho/para quinoid RF polymers are efficient for glucose oxidation and hydrogen peroxide reduction with concurrent 3,3',5,5'-tetramethylbenzidine oxidation under natural sunlight. The photoinduced colorimetric process could detect H2O2 up to 3.5 µM at 652 nm with the linear range of 0.1-2 mM. A limit of detection of 9.2 µM was exhibited by the system while measuring glucose with a linearity from 0.2 to 8.5 mM. The formation of hydroxyl radicals (•OH) from glucose oxidation reactions was evidenced by spin trapping electron paramagnetic resonance studies conducted herein. The results indicated that RF resins possessed strong intrinsic glucose oxidase and peroxidase (POx)-like activity under natural sunlight with promising storage and operation. This simple photozyme will definitely have potential uses in biomimetic solar-driven catalysis, bioenergy, and biomedicine.

Chemically modified carbon nitride-chitin-acetic acid hybrid as a metal-free bifunctional nanozyme cascade of glucose oxidase-peroxidase for "click off" colorimetric detection of peroxide and glucose.

Hybrid nanomaterials-based artificial enzymes with numerous utilities are necessary to develop future bionic devices in mimicking physiological processes. This paper demonstrates bifunctional enzyme mimicking roles of a metal-free nanozyme hybrid of chemically modified graphitic carbon nitride (MGCN), chitin and acetic acid (AcOH). The MGCN exhibited glucose oxidase-mimicking activity and chitin-AcOH mirrored peroxidase. MGCN-chitin-AcOH when in contact with glucose, oxidised glucose to gluconic acid and hydrogen peroxide (H2O2) while the chitin-AcOH decomposed the generated H2O2, as proved separately, by concurrent oxidation of 3,3',5,5'-tetramethylbenzidine (TMB). The super-sensitive colorimetric process produced linear regression equation for H2O2 as A = 0.00105C + 0.0630 (C:µM, R2 = 0.9961) with a detection limit of 0.052 µM, whereas for glucose, the linear relationship was A = 0.00084C + 0.0458 (C:µM, R2 = 0.9952) having a detection limit of 0.055 µM. The developed method was also successfully applied for assessment of H2O2 and glucose in human serum and urine samples. Non-enzymatic glucose test strips from MGCN-chitin-AcOH based hydrogel were reported and verified for semi-quantitative analysis of glucose. These compared well with results from standard enzyme-based colorimetric procedure. The developed hybrid nanozyme provided feasible alternatives to the two natural enzymes (peroxidase and glucose oxidase) realized through real sample analysis. The developed hybrid nanozyme can be successfully used for colorimetric detection of peroxide and glucose in medical diagnostics.

Three-dimensional graphene for electrochemical detection of Cadmium in Klebsiella michiganensis to study the influence of Cadmium uptake in rice plant.

Cadmium (Cd), as a hazardous pollutant present in the environment as well as within biological samples, needs to be detected and remediated at the same time. Although many types of Cd detection techniques have been developed globally, there is no evidence to analyse Cd2+ ion electrochemically using graphene-based electrode for bioaccumulation of Cd in bacteria and plants. The present study describes the fabrication and characterization of a three-dimensional reduced graphene oxide-based electrode to detect bioaccumulation of Cd within the bacterial cell and rice tissues applying differential pulse voltammetry (DPV) technique. In addition, X-ray fluorescence (XRF) and X-ray diffraction (XRD) studies were performed as supporting tools for this study in the selected Cd resistant plant growth promoting rhizobacterial (PGPR) strain, Klebsiella michiganensis MCC3089. This strain was characterized based on its plant growth promoting (PGP) traits and exhibited bioaccumulation of Cd both under high and low Cd concentrations, of which the latter is more environmentally significant. The Cd-sequestration ability of this strain was found to reduce Cd uptake within rice seedlings.

In-silico study of biotic and abiotic stress-related transcription factor binding sites in the promoter regions of rice germin-like protein genes.

Germin-like proteins (GLPs) are involved in biotic and abiotic stress tolerance in different plant species. Rice (Oryza sativa L.) genome contains about 40 GLP family member proteins in nine chromosomes. Although some of the rice GLP (OsGLP) promoters have been studied through in silico analysis as well as experimentally, studies regarding the distribution pattern of the biotic and abiotic stress associated transcription factor binding sites (TFbs) in the promoter regions of OsGLP genes have not been attempted thoroughly. Several transcription factors (TFs) namely NAC, WRKY, bHLH, bZIP, MYB and AP2/ERF act as major TFs concerned with biotic as well as abiotic stress responses across various plant species. In the present study the in silico analysis was carried out using the 1.5 kilobases (kb) promoter regions from 40 different OsGLP genes for the presence of NAC, WRKY, bHLH, bZIP, MYB and AP2/ERF TFbs in it. Among various OsGLP gene promoters, OsGLP8-11 was found to contain highest number of tested TFbs in the promoter region whereas the promoter region of OsGLP5-1 depicted least amount of TFbs. Phylogenetic study of promoter regions of different OsGLP genes revealed four different clades. Our analyses could reveal the evolutionary significance of different OsGLP gene promoters. It can be presumed from the present findings as well as previous reports that OsGLP gene duplications and subsequent variations in the TFbs in OsGLP gene promoter regions might be the consequences of neofunctionalization of OsGLP genes and their promoters for biotic and abiotic stress tolerance in rice.

3­Mercapto­propanoic acid modified cellulose filter paper for quick removal of arsenate from drinking water.

This paper reports a simple, facile and rapid preparation of 3­mercapto­propanoic acid (MPA) modified cellulose filter paper (MPA-Cell paper) for arsenate removal from drinking water. The MPA was covalently grafted to the cellulose filter paper (Cell) by esterification process through the formation of O­acylisourea intermediate and characterized by the FTIR, SEM, EDS and XPS analyses. The arsenate adsorption efficiency was studied for batch and semi-continuous systems while exploring the adsorption kinetics, isotherm and the effect of pH for the former. The experimental data fitted well with Langmuir, Dubinin-Radushkevich (DR) and pseudo second order kinetic models. The mechanism of adsorption was studied by FTIR spectroscopy utilizing the adsorption isotherm, kinetic model and XPS results. The modified filter paper performed well at nearly neutral pH in arsenate removal through adsorption and demonstrated a significant arsenate uptake capacity of 92.59 mg/g. The DR and FTIR results indicated that the adsorption of arsenate ion occurred through ion exchange process. The MPA-Cell paper could have a potential use as low-cost but efficient commercial adsorbent for arsenate abatement from contaminated drinking water by both batch as well as semi-continuous operating systems. The MPA-Cell paper could purify ground water containing high level of arsenate.

Enhancement of growth and salt tolerance of rice seedlings by ACC deaminase-producing Burkholderia sp. MTCC 12259.

Increasing soil salinity is often associated with accelerated ethylene production in plants, leading to overall growth reduction. The salt-tolerant 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing PGPR may alleviate salt stress by reducing the production of stress ethylene. In this study, a salt-tolerant ACC deaminase-producing strain named P50 was isolated from a coastal rice field in Odisha, India, which enhanced the growth of rice seedlings under salt stress. The P50 strain was identified as Burkholderia sp. based on phenotypic characteristics, MALDI-TOF MS data for ribosomal proteins and 16S rDNA sequence-based homology. Various PGP traits of strain P50 were characterized, among which the ACC deaminase activity was optimized at different physical conditions and confirmed by enzyme assay, as well as FTIR. The IAA, EPS and proline production of this strain were estimated under increasing NaCl concentrations essential for plant growth promotion under salt stress. Finally, the P50 strain was utilized in a gnotobiotic assay using rice seedlings (cv. Swarnamasuri) under saline stress. Seedlings treated with the P50 strain showed improvement in various morphological and biochemical characteristics, ROS scavenging antioxidant enzymatic activities, and reduced amounts of stress ethylene compared to non-inoculated strains under salinity. In addition, isolation of the ACC deaminase mutant of this strain was not found to reduce stress ethylene, confirming that the P50 strain was associated with a reduction in stress ethylene. Strain P50 was also found to colonize the root surfaces of rice seedlings associated with the plant-microbe interaction process. Thus, as an effective salt-tolerant PGPR, strain P50 can be utilized in salt-affected agricultural fields to improve plant growth in a sustainable manner.

The role of arsenic resistant Bacillus aryabhattai MCC3374 in promotion of rice seedlings growth and alleviation of arsenic phytotoxicity.

The biological agents have been utilized as an affordable alternative to conventional costly metal remediation technologies for last few years. The present investigation introduces arsenic (As) resistant plant growth promoting rhizobacteria (PGPR) isolated from the As-contaminated agricultural field of West Bengal, India that alleviates arsenic-induced toxicity and exhibited many plant growth promoting traits (PGP). The isolated strain designated as AS6 has identified as Bacillus aryabhattai based on phenotypic characteristics, physio-biochemical tests, MALDI-TOFMS bio-typing, FAME analysis and 16S rDNA sequence homology. The strain found to exhibit five times more resistance to arsenate than arsenite with minimum inhibitory concentrations (MIC) being 100 mM and 20 mM respectively. The result showed that accumulation of As was evidenced by SEM- EDAX, TEM-EDAX studies. The intracellular accumulation of arsenic was also confirmed as in bacterial biomass by AAS, FTIR, XRD and XRF analyses. The increased rate of As (V) reduction by this strain found to be exploited for the remediation of arsenic in the contaminated agricultural field. The strain also found to exhibit important PGP traits viz., ACC deaminase activity (2022 nmol α-ketobutyrate/mg protein/h), IAA production (166 µg/ml), N2 fixation (0.32 µgN fixed/h/mg proteins) and siderophore production (72%) etc. Positive influenced of AS6 strain on rice seedlings growth promotion under As stress was observed considering the several morphological, biochemical parameters including antioxidants activities as compared with an uninoculated set. Thus this strain might be exploited for stress amelioration and plant growth enhancement of rice cultivar under arsenic spiked agricultural soil.

Computational-based structural, functional and phylogenetic analysis of Enterobacter phytases.

Myo-inositol hexakisphosphate phosphohydrolases (i.e., phytases) are known to be a very important enzyme responsible for solubilization of insoluble phosphates. In the present study, Enterobacter phytases have characterized by different phylogenetic, structural and functional parameters using some standard bio-computational tools. Results showed that majority of the Enterobacter phytases are acidic in nature as most of the isoelectric points were under 7.0. The aliphatic indices predicted for the selected proteins were below 40 indicating their thermostable nature. The average molecular weight of the proteins was 48 kDa. The lower values of GRAVY of the said proteins implied that they have better interactions with water. Secondary structure prediction revealed that alpha-helical content was highest among the other forms such as sheets, coils, etc. Moreover, the predicted 3D structure of Enterobacter phytases divulged that the proteins consisted of four monomeric polypeptide chains i.e., it was a tetrameric protein. The predicted tertiary model of E. aerogenes (A0A0M3HCJ2) was deposited in Protein Model Database (Acc. No.: PM0080561) for further utilization after a thorough quality check from QMEAN and SAVES server. Functional analysis supported their classification as histidine acid phosphatases. Besides, multiple sequence alignment revealed that "DG-DP-LG" was the most highly conserved residues within the Enterobacter phytases. Thus, the present study will be useful in selecting suitable phytase-producing microbe exclusively for using in the animal food industry as a food additive.